Rey Castellanos, Natalia; Stashenko, Elena E.; Estévez Castro, Carlos F. (2015-09)
Abstract:The objective of this work was to evaluate DNA protective properties against UVR-induced genotoxicity of an L. origanoides extract obtained by CO2-supercritical fluid extraction and its major constituent, pinocembrin. L. origanoides extract composition was determined by gas chromatography-mass spectrometry (GC-MS) and its antigenotoxic effect using the SOS chromotest. Fluorescence microscopy analysis was also used in cell treatments in order to determine interferences with cell division and different nucleoid shapes categories were measured. The principal constituents of the plant extract were pinocembrin (54.9%), trans-β- caryophyllene (10.9%), carvacrol (6.3%), p-cimene (6.2%), thymol (4.6%), and 1,8-cineole (3.5%). L. origanoides extract protected bacterial cells against UVR-induced genotoxicity at doses between 0.016 and 1 mg/mL; this effect was related to its major constituent pinocembrin. Fluorescence microscopy analysis showed that pinocembrin modulated the cell division and nucleoid shape in E. coli cells. The results were discussed in relation to the DNA protective potential of the L. origanoides species.
Es proyecto?:no
Autor:Rey Castellanos, Natalia
Programa Nal. Colciencias:Programas de CT+I Ejecutados por Redes de Conocimiento
Insitución cofinanciadora:Departamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colciencias
Institución participante:Bio-Red-CO-CENIVAM
Tipo de producto resultado de investigación:info:eu-repo/semantics/other.generacióndecontenidos
Fecha:2015-09
Proyecto id:5507-543-31904
Nombre de proyecto principal:Programa: Bioprospección y desarrollo de ingredientes naturales para las industrias cosmética, farmacéutica y de productos de aseo con base en la biodiversidad colombiana
Comunidad vinculada:Comunidad científica colombiana